Abstract
Controlled studies on the immunopathogenesis of mucosal candidiasis in HIV infection have been hampered by the lack of a relevant animal model. We have previously reported that oral Candida infection in CD4C/HIV transgenic mice expressing gene products of HIV-1 in immune cells and developing an AIDS-like disease closely mimics oropharyngeal candidiasis in human HIV infection. The role of defective dendritic cells and CD4+ T cells in impaired induction of protective immunity and in the phenotype of chronic oral carriage of C. albicans can now be investigated under controlled conditions in these transgenic mice.
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References
Samaranayake LP. (1992) Oral mycoses in HIV infection. Oral Surg Oral Med Oral Pathol; 73(2):171–180.
Martins MD, Lozano-Chiu M, Rex JH. (1998) Declining rates of oropharyngeal candidiasis and carriage of Candida albicans associated with trends toward reduced rates of carriage of fluconazole-resistant C. albicans in human immunodeficiency virus-infected patients. Clin Infect Dis; 27(5):1291–1294.
de Repentigny L, Lewandowski D, Jolicoeur P. (2004) Immunopathogenesis of oropharyngeal candidiasis in human immunodeficiency virus infection. Clin Microbiol Rev; 17(4):729–759.
de Repentigny L, Aumont F, Ripeau JS, et al. (2002) Mucosal candidiasis in transgenic mice expressing human immunodeficiency virus type 1. J Infect Dis; 185(8):1103–1114.
Marquis M, Lewandowski D, Dugas V, et al. (2006) CD8+ T cells but not polymorphonuclear leukocytes are required to limit chronic oral carriage of Candida albicans in transgenic mice expressing human immunodeficiency virus type 1. Infect Immun; 74(4):2382–2391.
Lewandowski D, Marquis M, Aumont F, et al. (2006) Altered CD4+ T cell phenotype and function determine the susceptibility to mucosal candidiasis in transgenic mice expressing HIV-1. J Immunol; 177(1):479–491.
Hanna Z, Kay DG, Rebai N, Guimond A, Jothy S, Jolicoeur P. (1998) Nef harbors a major determinant of pathogenicity for an AIDS-like disease induced by HIV-1 in transgenic mice. Cell; 95:163–175.
Lacasse M, Fortier C, Trudel L, Collet AJ, Deslauriers N. (1990) Experimental oral candidosis in the mouse: microbiologic and histologic aspects. J Oral Pathol Med; 19(3):136–141.
Inaba K, Inaba M, Romani N, et al. (1992) Generation of large numbers of dendritic cells from mouse bone marrow cultures supplemented with granulocyte/macrophage colony-stimulating factor. J Exp Med; 176(6):1693–1702.
Poudrier J, Weng X, Kay DG, Hanna Z, Jolicoeur P. (2003) The AIDS-like disease of CD4C/human immunodeficiency virus transgenic mice is associated with accumulation of immature CD11bHi dendritic cells. J Virol; 77(21):11733–11744.
d’Ostiani CF, Del Sero G, Bacci A, et al. (2000) Dendritic cells discriminate between yeasts and hyphae of the fungus Candida albicans. Implications for initiation of T helper cell immunity in vitro and in vivo. J Exp Med; 191(10):1661–1674.
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de Repentigny, L., Lewandowski, D., Aumont, F., Hanna, Z., Jolicoeur, P. (2009). Oral Mucosal Cell Response to Candida albicans in Transgenic Mice Expressing HIV-1. In: Rupp, S., Sohn, K. (eds) Host-Pathogen Interactions. Methods in Molecular Biology, vol 470. Humana Press. https://doi.org/10.1007/978-1-59745-204-5_25
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DOI: https://doi.org/10.1007/978-1-59745-204-5_25
Publisher Name: Humana Press
Print ISBN: 978-1-58829-886-7
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