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Host-Pathogen Interactions pp 169–185Cite as

Antibody-Based Strategy to Identify Candida albicans Genes Expressed During Infections

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 470))

Abstract

Investigators have long used antibody-based screening strategies to identify Candida albicans immunogenic proteins and the genes that encode them during infections. With the recent availability of the C. albicans genome sequence and the development of genomic and proteomic technologies, it is now possible to efficiently conduct large-scale screening in standard research labs. C. albicans proteins and genes identified with a variety of screening methods have been implicated as important determinants of candidal virulence and exploited as vaccine and therapeutic targets. In this chapter, we describe methods used in our lab, in which sera recovered from patients with candidiasis are used to screen a C. albicans genomic DNA expression library. Immunoreactive colonies are detected by reaction with anti-human immunoglobulin, and the corresponding open reading frames are identified using the genome sequence database. The methods are also suitable for use with cDNA expression libraries, and they are complementary to proteomic screening strategies described elsewhere in this volume.

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Author information

Authors and Affiliations

  1. University of Florida College of Medicine and North Florida/South Georgia Veterans Health System, Gainesville, Florida, USA

    Cornelius J. Clancy

  2. Julius-Maximilians-University Würzburg, Department of Medicine, Division of Infectious Disease, University of Florida, Gainesville, Florida, USA

    Shaoji Cheng

  3. College of Medicine, University of Florida, Gainesville, Florida, USA

    M. Hong Nguyen

Authors
  1. Cornelius J. Clancy
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  2. Shaoji Cheng
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  3. M. Hong Nguyen
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Editor information

Editors and Affiliations

  1. Fraunhofer Institute for Interfacial Engineering and Biotechnology, Nobelstrasse 12, 70569 Stuttgart, Germany

    Steffen Rupp  & Kai Sohn  & 

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Clancy, C.J., Cheng, S., Nguyen, M.H. (2009). Antibody-Based Strategy to Identify Candida albicans Genes Expressed During Infections. In: Rupp, S., Sohn, K. (eds) Host-Pathogen Interactions. Methods in Molecular Biology, vol 470. Humana Press. https://doi.org/10.1007/978-1-59745-204-5_14

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  • DOI: https://doi.org/10.1007/978-1-59745-204-5_14

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-886-7

  • Online ISBN: 978-1-59745-204-5

  • eBook Packages: Springer Protocols

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