Abstract
The microinjection of mRNA that is transcribed and capped in vitro into fertilized eggs and embryos of Xenopus laevis provides a powerful means for discovering the function of proteins during early development. Proteins may be overexpressed for a gain-of-function effect or exogenous protein function may be compromised by the microinjection of mRNA encoding “dominant-negative” proteins. This methodology is particularly suited for the investigation of the regulation of the cell cycle, checkpoints, and apoptosis in early development.
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Sible, J.C., Wroble, B.N. (2009). Expression of Exogenous mRNA in Xenopus laevis Embryos for the Study of Cell Cycle Regulation. In: Carroll, D. (eds) Microinjection. Methods in Molecular Biology, vol 518. Humana Press. https://doi.org/10.1007/978-1-59745-202-1_1
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DOI: https://doi.org/10.1007/978-1-59745-202-1_1
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