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Hepatocyte Transplantation pp 17–23Cite as

Isolation of Human Hepatocytes

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 481))

Abstract

Protocols for isolation of human hepatocytes have been developed. The isolated cells can be used not only in research but also for transplantation in patients with liver disease, especially acute liver failure and liverbased metabolic/synthetic conditions. The aim of hepatocyte transplantation is to correct the missing liver function(s) and allow either the recovery of the liver or buy the patient time until a suitable donor liver is available for transplantation.

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References

  1. Fisher, R. A., Strom, S. C. (2006) Human hepatocyte transplantation: worldwide results. Transplantation 82, 441–449.

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  5. Mitry, R. R., Hughes, R. D., Aw, M. M., et al. (2003) Human hepatocyte isolation and relationship of cell viability to early graft function. Cell Transpl 12, 69–74.

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  6. Freshney, R. I. (2000) Culture of Animal Cells. Wiley-Liss, New York, NY, pp. 309–328.

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Authors
  1. Ragai R. Mitry
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Editor information

Editors and Affiliations

  1. King’s College Hospital, London, UK

    Anil Dhawan

  2. School of Medicine, King’s College London, London, UK

    Robin D. Hughes

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Cite this protocol

Mitry, R. (2009). Isolation of Human Hepatocytes. In: Dhawan, A., Hughes, R. (eds) Hepatocyte Transplantation. Methods in Molecular Biology, vol 481. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-201-4_2

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  • DOI: https://doi.org/10.1007/978-1-59745-201-4_2

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-172-1

  • Online ISBN: 978-1-59745-201-4

  • eBook Packages: Springer Protocols

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