Abstract
With the advent of “proteomics,” many previously unidentified proteins will be isolated in gels for subsequent structural and functional characterization. It is, therefore, important that methods are available for detecting these proteins with minimal risk of modification. This chapter describes a “reverse” staining technique that facilitates the sensitive detection of unmodified proteins (1–9).
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Acknowledgments
The author expresses his gratitude to his former colleagues, Drs. L. Castellanos-Serra and E. Hardy from the Centre for Genetic Engineering and Biotechnology (CIGB), Havana, Cuba for their contributions to the development of the reverse staining concept, and to the CIGB for early support. Currently, the author is a New Investigator of the Canadian Institutes of Health Research and the Heart and Stroke Foundation of Canada as well as funded by grants from Natural Sciences and Engineering Council and the Canadian Institutes of Health Research.
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Fernandez-Patron, C. (2009). Zn2+-Reverse Staining Technique. In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-198-7_46
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DOI: https://doi.org/10.1007/978-1-59745-198-7_46
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