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Separation of Proteins by Blue Native Electrophoresis (BN-PAGE)

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Abstract

Proteins often work together in protein complexes. For the analysis of the function as well as the structure of proteins it is often of interest to analyze the assembly of such complexes. While protein complexes normally dissociate into their subunits when analyzed by SDS-PAGE (Fig. 1B), nondenaturing electrophoresis (so called native electrophoresis) methods leave the complexes intact. Blue native polyacrylamide gel electrophoresis (BN-PAGE, Fig. 1C), a method developed by Schägger and Jagow (1), has proven to be a valuable tool to analyze the respiratory chain complexes of different organisms (2, 3) to investigate the complexes of the import machinery of mitochondria (4–6) or chloroplasts (7), or to analyze the proteome of isolated organelles or whole cellular lysates (8, 9).

Keywords

  • Respiratory Chain Complex
  • Cellular Lysate
  • Import Machinery
  • Cathode Buffer
  • Native Electrophoresis

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Dian, E.A., Rassow, J., Motz, C. (2009). Separation of Proteins by Blue Native Electrophoresis (BN-PAGE). In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-198-7_24

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  • DOI: https://doi.org/10.1007/978-1-59745-198-7_24

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60327-474-6

  • Online ISBN: 978-1-59745-198-7

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