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Plant Protein Sample Preparation for 2-DE

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The Protein Protocols Handbook

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

Most plant tissues are not a ready source for protein extraction and need specific precautions. The cell wall and the vacuole make up the majority of the cell mass, with the cytosol representing only 1 to 2 % of the total cell volume. Subsequently, plant tissues have a relatively low protein content compared to bacterial or animal tissues. The cell wall and the vacuole are associated with numerous substances responsible for irreproducible results such as proteolytic breakdown, streaking and charge heterogeneity. Most common interfering substances are phenolic compounds, proteolytic and oxidative enzymes, terpenes, pigments, organic acids, inhibitory ions, and carbohydrates.

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Acknowledgment

Dr. S.C. Carpentier is supported by a postdoctoral fellowship of the K.U.Leuven.

Author information

Authors and Affiliations

  1. Faculty of Bioscience Engineering, Division of Crop Biotechnics, Leuven, Belgium

    Sebastien Christian Carpentier

  2. Department of Biosystems, K.U.Leuven, Faculty of Bioscience Engineering, Leuven, Belgium

    Rony Swennen & Bart Panis

Authors
  1. Sebastien Christian Carpentier
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  2. Rony Swennen
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  3. Bart Panis
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Editor information

Editors and Affiliations

  1. School of Life Sciences, University of Hertfordshire, Hatfield, Hertfordshire, UK

    John M. Walker

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© 2009 Springer Science+Business Media, LLC

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Carpentier, S.C., Swennen, R., Panis, B. (2009). Plant Protein Sample Preparation for 2-DE. In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-198-7_13

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  • DOI: https://doi.org/10.1007/978-1-59745-198-7_13

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60327-474-6

  • Online ISBN: 978-1-59745-198-7

  • eBook Packages: Springer Protocols

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