References
Klose, J. and Kobalz, U. (1995) Two-dimensional electrophoresis of proteins: an updated protocol and implications for a functional analysis of the genome. Electro-phoresis 16, 1034–1059.
Challapalli, K. K., Zabel, C., Schuchhardt, J., Kaindl, A. M., Klose, J., and Herzel, H. (2004) High reproducibility of large-gel two-dimensional electrophoresis. Electrophoresis 25, 3040–3047.
Klose, J. (1999) Fractionated extraction of total tissue proteins from mouse and human for 2-D electrophoresis. Meth. Mo.l Biol. 112, 67–85.
Zabel, C., Sagi, D., Kaindl, A. M., Steireif, N., Klare, Y., Mao, L., Peters, H., Wacker, M. A., Kleene, R., and Klose, J. (2006) Comparative proteomics in neu-rodegenerative and non-neurodegenerative diseases suggest nodal point proteins in regulatory networking. J Proteome Res. 5, 1948–1958.
Acknowledgment
The authors appreciate critical comments and support from Marion Herrmann and Yvonne Kläre.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Zabel, C., Klose, J. (2009). Mouse and Human Tissue Sample Preparation for 2-D Electrophoresis. In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-198-7_12
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DOI: https://doi.org/10.1007/978-1-59745-198-7_12
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