Summary
The production of recombinant proteins usually involves the exploration of a wide variety of expression and purification methodologies in the pursuit of a strategy tailored to a particular protein. The methods applied are reliant on exploiting individual differences between expression systems or the variations in specific protein properties. These bespoke strategies have not lent themselves to high-throughput methodologies. Ultimately the development of robust generic methods capable of simplifying and stabilizing the process, allowing automation, was necessary to increase throughput. This chapter describes a series of high-throughput methods used to express, purify, and quantify recombinant protein produced in E. coli or insect cells.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Chambers, S.P., Fulghum, J.R., Austen, D.A., Lu, F., Swalley, S.E. (2009). E. coli and Insect Cell Expression, Automated Purification and Quantitative Analysis. In: Doyle, S.A. (eds) High Throughput Protein Expression and Purification. Methods in Molecular Biology, vol 498. Humana Press. https://doi.org/10.1007/978-1-59745-196-3_10
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DOI: https://doi.org/10.1007/978-1-59745-196-3_10
Publisher Name: Humana Press
Print ISBN: 978-1-58829-879-9
Online ISBN: 978-1-59745-196-3
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