Abstract
Bleomycin and Interleukin 12 have been used clinically to treat tumors; however, the co-administration of Bleomycin and Interleukin 12 followed by electroporation has not been tested clinically. In this study, dogs with spontaneous head and neck tumors were treated with one co-administration of Bleomycin and Interleukin 12 plasmid DNA followed by electroporation. The regression of the recurrent papillary tumor and the adjacent metastatic bone tumor was analyzed by multiple CT scans. The papillary tumor was completely eradicated in less than 2 weeks, and the bone tumor was not visible 23 weeks after the administration.
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References
1. Dorr, R.T. and Von- Huff, D.D. (1994) Drug monographs. In: Dorr, R.T. and Von-Huff, D.D., (eds.). Cancer chemotherapy handbook, Appleton and Lange, Norwalk, CT, pp. 227–236.
2. Berger R.M., Projan S.J., Horwitz S.B., and Peisach J. (1986) The DNA cleavage mechanism of iron-bleomycin. J. Biol. Chem. 261, 15955–15959.
Perry M. (ed.) (2001) The chemotherapy source book. Lippincott Williams & Wilkins, Philadelphia, PA.
Chabner B.A. and Longo D.L. (eds.) (2001) Cancer chemotherapy and biotherapy. Lippincott, Williams, and Wilkins, Philadelphia, PA.
5. Poddevin B., Orlowski S., Belehradek, J., Jr., and Mir L.M. (1991) Very high toxicity of bleomycin introduced into the cytosol of cells in culture. Biochem. Pharmacol. 42, S67–S75.
6. Trinchieri G. (2003) Interleukin-12 and the regulation of innate resistance and adaptive immunity. Nat. Rev. Immunol. 3, 133–146.
7. Robertson M.J., Cameron C., Atkins M.B., et al. (1999) Immunological effects of Interleukin 12 administered by bolus intravenous injection to patients with cancer. Clin. Cancer Res. 5, 9–16.
8. Motzer R.J., Rakhit A., Schwartz L.H., et al. (1998) Phase I trial of subcutaneous recombinant human Interleukin-12 in patients with advance renal cell carcinoma. Clin. Cancer Res. 4, 1183–1191.
9. Li S., Zhang X. and Xia X. (2002) Regression of tumor growth and induction of long-term antitumor memory by Interleukin 12 electrogenetherapy. J. Natl. Cancer Inst. 94, 668–675.
10. Torrero M.N., Henk W.G., and Li S. (2006) Regression of high-grade malignancy in mice by bleomycin and Interleukin-12 electrochemogenetherapy. Clin. Cancer Res. 12, 257–263.
11. Coleman M., Muller S., Quezada A., et al. (1998) Nonviral interferon alpha gene therapy inhibits growth of established tumors by eliciting a systemic response. Hum. Gene Ther. 9, 2223–2230.
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Cutrera, J., Torrero, M., Shiomitsu, K., Mauldin, N., Li, S. (2008). Intratumoral Bleomycin and IL-12 Electrochemogenetherapy for Treating Head and Neck Tumors in Dogs. In: Li, S. (eds) Electroporation Protocols. Methods in Molecular Biology™, vol 423. Humana Press. https://doi.org/10.1007/978-1-59745-194-9_24
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DOI: https://doi.org/10.1007/978-1-59745-194-9_24
Publisher Name: Humana Press
Print ISBN: 978-1-58829-877-5
Online ISBN: 978-1-59745-194-9
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