Summary
RNA interference (RNAi) is a powerful tool in deciphering gene function. It has been used extensively, especially in cultured mammalian cells. We have shown that RNAi-induced gene silencing can be generated in mice. With conventional transgenic techniques, shRNA-expressing constructs can be introduced into one-cell mouse embryos. The transgenic animals so obtained exhibit reduced expression of the targeted genes. Furthermore, the knockdown effect can be transmitted through the germline in these animals. We describe a method of generating a transgenic RNAi mouse line.
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Acknowledgments
The author would like to thank Dr. S. Peng for technical help. This work was supported by Grants DK063964 and EY014745 from the National Institutes of Health.
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© 2008 Humana Press, a part of Springer Science+Business Media, LLC
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Zhang, P. (2008). Transgenic RNA Interference in Mice. In: Barik, S. (eds) RNAi. Methods in Molecular Biology™, vol 442. Humana Press. https://doi.org/10.1007/978-1-59745-191-8_18
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DOI: https://doi.org/10.1007/978-1-59745-191-8_18
Publisher Name: Humana Press
Print ISBN: 978-1-58829-874-4
Online ISBN: 978-1-59745-191-8
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