Summary
An efficient RNAi largely depends on optimal design of the siRNA. In recent studies, Dicer substrates were found to be more potent than classical synthetic 21-mer siRNAs, suggesting a coupling of the Dicer-mediated processing step to the efficient assembly of the silencing complex, RISC. We describe the fundamental principles and experimental results leading to optimal Dicer substrates.
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© 2008 Humana Press, a part of Springer Science+Business Media, LLC
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Amarzguioui, M., Rossi, J.J. (2008). Principles of Dicer Substrate (D-siRNA) Design and Function. In: Barik, S. (eds) RNAi. Methods in Molecular Biology™, vol 442. Humana Press. https://doi.org/10.1007/978-1-59745-191-8_1
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DOI: https://doi.org/10.1007/978-1-59745-191-8_1
Publisher Name: Humana Press
Print ISBN: 978-1-58829-874-4
Online ISBN: 978-1-59745-191-8
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