Preparation and Analysis of Uniquely Positioned Mononucleosomes

  • Daria A. Gaykalova
  • Olga I. Kulaeva
  • Vladimir A. Bondarenko
  • Vasily M. Studitsky
Part of the Methods in Molecular Biology book series (MIMB, volume 523)


Short DNA fragments containing single, uniquely positioned nucleosome cores have been extensively employed as simple model experimental systems for analysis of many intranuclear processes, including binding of proteins to nucleosomes, transcription, DNA repair and ATP-dependent chromatin remodeling. In many cases such simple model templates faithfully recapitulate numerous important aspects of these processes. Here we describe several recently developed procedures for obtaining and analysis of mononucleosomes that are uniquely positioned on 150–600 bp DNA fragments.

Key words

Positioned nucleosome cores chromatin reconstitution 



We thank John Widom for plasmids containing the nucleosome-positioning sequences. This work was supported by NIH grant GM58650 to V.M.S.


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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Daria A. Gaykalova
    • 1
  • Olga I. Kulaeva
    • 1
  • Vladimir A. Bondarenko
    • 1
  • Vasily M. Studitsky
    • 1
  1. 1.Department of PharmacologyRobert Wood Johnson Medical School, University of Medicine and Dentistry of New JerseyPiscatawayUSA

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