Abstract
Defining protein-protein interaction networks is a major goal of proteomics. Here, we present a protocol for coimmunoprecipitation, a technique suitable for the isolation of whole protein complexes in vivo and their subsequent identification by either immunoblotting or mass spectrometric sequencing combined to database search.
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Acknowledgments
This work was supported by the Austrian Genome Network GEN-AU/ APP-II of the Austrian Ministry for Education, Science and Culture and by the OeNB (Pr.Nr. 10855).
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© 2008 Humana Press, a part of Springer Science+Business Media, LLC
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Falsone, S.F., Gesslbauer, B., Kungl, A.J. (2008). Coimmunoprecipitation and Proteomic Analyses. In: Starkey, M., Elaswarapu, R. (eds) Genomics Protocols. Methods in Molecular Biology™, vol 439. Humana Press. https://doi.org/10.1007/978-1-59745-188-8_20
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DOI: https://doi.org/10.1007/978-1-59745-188-8_20
Publisher Name: Humana Press
Print ISBN: 978-1-58829-871-3
Online ISBN: 978-1-59745-188-8
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