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Transmigration of Human CD34+ Cells

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Hematopoietic Stem Cell Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 430))

Summary

Understanding mechanisms responsible for engraftment of hematopoietic stem cells (HSC) is important to achieve successful HSC transplantation. Homing of HSC to the bone marrow niche is believed to be a crucial step for engraftment. However, the molecular mechanisms that regulate HSC homing are not understood well. Migration of HSC in response to cytokines and chemokines is believed to be one of the critical steps of HSC homing and mobilization. Evaluating the migration of HSC will help to understand the mechanisms responsible for their homing and/or mobilization. In this chapter, we will describe the methodology utilized in our laboratory to evaluate migration of human CD34+ cells that contain HSC.

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Author information

Authors and Affiliations

  1. Department of Microbiology and Immunology and the Walther Oncology Center, Indiana University School of Medicine, and the Walther Cancer Institute, Indianapolis, IN

    Seiji Fukuda & Louis M. Pelus

Authors
  1. Seiji Fukuda
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  2. Louis M. Pelus
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Editor information

Editors and Affiliations

  1. Department of Medicine, Division of Hematology & Oncology, Center for Stem Cell and Regenerative Medicine, Case Western Reserve University, Cleveland, OH, USA

    Kevin D. Bunting

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© 2008 Humana Press, a part of Springer Science+Business Media, LLC

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Fukuda, S., Pelus, L.M. (2008). Transmigration of Human CD34+ Cells. In: Bunting, K.D. (eds) Hematopoietic Stem Cell Protocols. Methods in Molecular Biology™, vol 430. Humana Press. https://doi.org/10.1007/978-1-59745-182-6_4

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  • DOI: https://doi.org/10.1007/978-1-59745-182-6_4

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-868-3

  • Online ISBN: 978-1-59745-182-6

  • eBook Packages: Springer Protocols

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