Summary
Endothelial exocytosis of granules is a rapid response to vascular injury. However, the molecular machinery that regulates exocytosis in endothelial cells is not well understood. Recently developed techniques have defined the endothelial proteins that control vesicle and granule trafficking in endothelial cells. These techniques have revealed that syntaxin 4, synaptobrevin 3, and N-ethylmaleimide-sensitive factor (NSF) play a critical role in endothelial granule exocytosis. Additional studies have shown that nitric oxide regulates exocytosis by chemically modifying NSF. Further characterization of the factors that regulate exocytosis will lead to novel treatments for vascular diseases such as myocardial infarction and stroke.
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Acknowledgments
We would like to thank Ms. Jacqueline Hewitt for assistance preparing the manuscript. Supported was received by grants from the National Institutes of Health (P01 HL56091, R01 HL074061, R01 HL78635, P01 HL65608), AHA (EIG 0140210N), the Ciccarone Center, the John and Cora H. Davis Foundation, and the Clarence Doodeman Professorship to C.J.L. and by grants RR07002 and HL074945 from the National Institutes of Health to C.M.
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© 2008 Humana Press, a part of Springer Science+Business Media, LLC
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Yamakuchi, M. et al. (2008). Exocytosis of Endothelial Cells Is Regulated by N-Ethylmaleimide-Sensitive Factor. In: Ivanov, A.I. (eds) Exocytosis and Endocytosis. Methods in Molecular Biology, vol 440. Humana Press. https://doi.org/10.1007/978-1-59745-178-9_15
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DOI: https://doi.org/10.1007/978-1-59745-178-9_15
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