Summary
The plasma membrane of epithelial cells has two physically separated membrane domains. This membrane polarization is essential for the function of epithelial cells. It has been well established that different plasma membrane syntaxin forms are expressed in epithelial cells. In addition, these syntaxin forms can have a polarized localization, suggesting that they may play a direct role in the specificity of polarized membrane delivery. To determine the mechanism of the polarized syntaxin localization, we have made several chimeras of syntaxin 3 and 4. This allowed us to identify the protein sequences involved in this polarized localization. Using this technique, we showed that targeting information of syntaxin 3 and 4 is located in the first 30 amino acids.
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Acknowledgements
I would like to thank Mirjam Zegers for critically reading the manuscript. This work was supported by a Ohio Division Supported Research Grant of the ACS.
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© 2008 Humana Press, a part of Springer Science+Business Media, LLC
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ter Beest, M.B. (2008). The Use of Syntaxin Chimeras to Study Polarized Protein Trafficking in Epithelial Cells. In: Ivanov, A.I. (eds) Exocytosis and Endocytosis. Methods in Molecular Biology, vol 440. Humana Press. https://doi.org/10.1007/978-1-59745-178-9_13
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DOI: https://doi.org/10.1007/978-1-59745-178-9_13
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