Abstract
In HIV-1, tRNALys3 serves as the primer for reverse transcription of minus strand strong stop cDNA. During viral assembly, the tRNALys isoacceptors, tRNALys1,2 and tRNALys3, are selectively packaged into the virion. The selectively packaging of tRNALys3 facilitates the annealing of tRNALys3 to the viral genome and the initiation of reverse transcription. We describe herein a set of experimental approaches for studying the mechanism by which tRNALys is selectively incorporated into HIV-1 and investigate how primer tRNALys3 is annealed to viral genome. The methods described will also help in the analysis of cellular RNAs packaged in the virus particles.
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Cen, S., Guo, F., Kleiman, L. (2009). Methods for Analysis of Incorporation and Annealing Methods for Analysis of tRNALys in HIV-1. In: Prasad, V.R., Kalpana, G.V. (eds) HIV Protocols. Methods In Molecular Biology™, vol 485. Humana Press. https://doi.org/10.1007/978-1-59745-170-3_16
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DOI: https://doi.org/10.1007/978-1-59745-170-3_16
Publisher Name: Humana Press
Print ISBN: 978-1-58829-859-1
Online ISBN: 978-1-59745-170-3
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