Abstract
The management of clone-bearing recipients and neonatal clones is a critical component of successful cloning of mammals by nuclear transfer. The methodology discussed in this chapter is based largely on a double corticosteroid regime to induce parturition and fetal organ maturation in bovine clones. Lung maturation, particularly, is a major factor in clone calf viability. The animal health care involved in maximizing clone survival begins at embryo transfer and continues for the life of that animal.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Wilmut, I., Beaujean, N., De Sousa, P. A., et al. (2002) Somatic cell nuclear transfer. Nature 419, 583–587.
Garry, F. B., Adams, R., McCann, J. P., and Odde, K. G. (1996) Postnatal characteristics of calves produced by nuclear transfer cloning. Theriogenology 45, 141–152.
Hill, J. R., Roussel, A. J., Cibelli, J. B., et al. (1999) Clinical and pathologic features of cloned transgenic calves and foetuses (13 case studies). Theriogenology 51, 1451–1465.
Chavatte-Palmer, P., Heyman, Y., Richard, C., et al. (2002) Clinical, hormonal, and hematologic characteristics of bovine calves derived from nuclei from somatic cells. Biol Reprod 66, 1596–1603.
Hill, J. R. and Chavatte-Palmer, P. (2002) Pregnancy and Neonatal Care of Cloned Animals, in Principles of Cloning (Cibelli, J., Lanza, R. P., Campbell, K. H.S., and West, M. D., eds.) Elsevier Science, New York pp 247–266.
Sasser, R. G., Ruder, C. A., Ivani, K. A., Butler, J. E., and Hamilton, W. C. (1986) Detection of prenancy by radioimmunoassay of a novel pregnancy-specific protein in serum of cows and a profile of serum concentrations during gestation. Biol. Reprod. 35 936–942.
Mialon, M. M., Camous, S., Renand, G., Martal, J., and Menissier, F. (1993) Peripheral concentration of a 60-kDa pregnancy specific protein during gestation and after calving in relationship to embryonic mortality in cattle. Reprod. Nutr. Dev. 33 269–282.
Zoli, A. P., Guilbault, L. A., Delahaut, P., Benitez-Ortiz, W., and Beckers, J. F. (1992) Radioimmunoassay of a bovine pregnancy associated glycoprotein in serum: Its application for pregnancy diagnosis. Biol. Reprod. 46 83–92.
Hill, J. R., Burghardt, R. C., Jones, K., et al. (2000) Evidence for placental abnormality as the major cause of mortality in first-trimester somatic cell cloned bovine foetuses. Biol. Reprod. 63, 1787–1794.
Shiels, P. G., Kind, A. J., Campbell, K. H., et al. (1999) Analysis of telomere lengths in cloned sheep. Nature 399, 316–317.
Clark, A. J., Ferrier, P., Aslam, S., et al. (2003) Proliferative lifespan is conserved after nuclear transfer. Nat. Cell Biol. 5, 535–538.
Ogura, A., Inoue, K., Ogonuki, N., Lee, J., Kohda, T., and Ishino, F. (2002) Phenotypic effects of somatic cell cloning in the mouse. Cloning Stem Cells 4, 397–405.
Shimozawa, N., Ono, Y., Kimoto, S., et al. (2002) Abnormalities in cloned mice are not transmitted to the progeny. Genesis 34, 203–207.
Wells, D. N. (2003) Cloning in livestock agriculture. Reprod. Suppl. 61, 131–150.
Wilson, J. M., Williams, J. D., Bondioli, K. R., Looney, C. R., Westhusin, M. E., and McCalla, D. F. (1995) Comparison of birth weight and growth characteristics of bovine calves produced by nuclear transfer (cloning), embryo transfer and natural mating. Anim. Reprod. Sci. 38, 73–83.
Morrow, C. J. and Berg, M. C. (2003) Ultrasound guided transvaginal collection of amniotic and/or allantoic fluid from cattle. Theriogenology 59, 539.
Hill, J. R., Schlafer, D. H., Fisher, P. J., and Davies, C. J. (2002) Abnormal expression of trophoblast major histocompatibility complex class I antigens in cloned bovine pregnancies is associated with a pronounced endometrial lymphocytic response. Biol. Reprod. 67, 55–63.
Eigenmann, U. J. E., Schoon, H. A., Jahn, D., and Grunert, E. (1984) Neonatal respiratory distress syndrome in the calf. Vet. Rec. 114, 141–144.
Zaremba, W., Grunert, E., and Aurich, J. E. (1997) Prophylaxis of respiratory distress syndrome in premature calves by administration of dexamethasone or a prostaglandin F2α analogue to their dams before parturition. Am. J. Vet. Res. 58, 404–407.
Renard, J. P., Chastant, S., Chesne, P., et al. (1999) Lymphoid hypoplasia and somatic cloning. Lancet 353, 1489–1491.
Lanza, R. P., Cibelli, J. B., Faber, D., et al. (2001) Cloned cattle can be healthy and normal. Science 294, 1893–1894.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2006 Humana Press Inc.
About this protocol
Cite this protocol
Forsyth, J.T., Wells, D.N. (2006). Health and Neonatal Care of Bovine Clones. In: Verma, P.J., Trounson, A.O. (eds) Nuclear Transfer Protocols. Methods in Molecular Biology™, vol 348. Humana Press. https://doi.org/10.1007/978-1-59745-154-3_6
Download citation
DOI: https://doi.org/10.1007/978-1-59745-154-3_6
Publisher Name: Humana Press
Print ISBN: 978-1-58829-280-3
Online ISBN: 978-1-59745-154-3
eBook Packages: Springer Protocols