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Health and Neonatal Care of Bovine Clones

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Book cover Nuclear Transfer Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 348))

Abstract

The management of clone-bearing recipients and neonatal clones is a critical component of successful cloning of mammals by nuclear transfer. The methodology discussed in this chapter is based largely on a double corticosteroid regime to induce parturition and fetal organ maturation in bovine clones. Lung maturation, particularly, is a major factor in clone calf viability. The animal health care involved in maximizing clone survival begins at embryo transfer and continues for the life of that animal.

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References

  1. Wilmut, I., Beaujean, N., De Sousa, P. A., et al. (2002) Somatic cell nuclear transfer. Nature 419, 583–587.

    Article  CAS  PubMed  Google Scholar 

  2. Garry, F. B., Adams, R., McCann, J. P., and Odde, K. G. (1996) Postnatal characteristics of calves produced by nuclear transfer cloning. Theriogenology 45, 141–152.

    Article  Google Scholar 

  3. Hill, J. R., Roussel, A. J., Cibelli, J. B., et al. (1999) Clinical and pathologic features of cloned transgenic calves and foetuses (13 case studies). Theriogenology 51, 1451–1465.

    Article  CAS  PubMed  Google Scholar 

  4. Chavatte-Palmer, P., Heyman, Y., Richard, C., et al. (2002) Clinical, hormonal, and hematologic characteristics of bovine calves derived from nuclei from somatic cells. Biol Reprod 66, 1596–1603.

    Article  CAS  PubMed  Google Scholar 

  5. Hill, J. R. and Chavatte-Palmer, P. (2002) Pregnancy and Neonatal Care of Cloned Animals, in Principles of Cloning (Cibelli, J., Lanza, R. P., Campbell, K. H.S., and West, M. D., eds.) Elsevier Science, New York pp 247–266.

    Chapter  Google Scholar 

  6. Sasser, R. G., Ruder, C. A., Ivani, K. A., Butler, J. E., and Hamilton, W. C. (1986) Detection of prenancy by radioimmunoassay of a novel pregnancy-specific protein in serum of cows and a profile of serum concentrations during gestation. Biol. Reprod. 35 936–942.

    Article  CAS  PubMed  Google Scholar 

  7. Mialon, M. M., Camous, S., Renand, G., Martal, J., and Menissier, F. (1993) Peripheral concentration of a 60-kDa pregnancy specific protein during gestation and after calving in relationship to embryonic mortality in cattle. Reprod. Nutr. Dev. 33 269–282.

    Article  CAS  PubMed  Google Scholar 

  8. Zoli, A. P., Guilbault, L. A., Delahaut, P., Benitez-Ortiz, W., and Beckers, J. F. (1992) Radioimmunoassay of a bovine pregnancy associated glycoprotein in serum: Its application for pregnancy diagnosis. Biol. Reprod. 46 83–92.

    Article  CAS  PubMed  Google Scholar 

  9. Hill, J. R., Burghardt, R. C., Jones, K., et al. (2000) Evidence for placental abnormality as the major cause of mortality in first-trimester somatic cell cloned bovine foetuses. Biol. Reprod. 63, 1787–1794.

    Article  CAS  PubMed  Google Scholar 

  10. Shiels, P. G., Kind, A. J., Campbell, K. H., et al. (1999) Analysis of telomere lengths in cloned sheep. Nature 399, 316–317.

    Article  CAS  PubMed  Google Scholar 

  11. Clark, A. J., Ferrier, P., Aslam, S., et al. (2003) Proliferative lifespan is conserved after nuclear transfer. Nat. Cell Biol. 5, 535–538.

    Article  CAS  PubMed  Google Scholar 

  12. Ogura, A., Inoue, K., Ogonuki, N., Lee, J., Kohda, T., and Ishino, F. (2002) Phenotypic effects of somatic cell cloning in the mouse. Cloning Stem Cells 4, 397–405.

    Article  CAS  PubMed  Google Scholar 

  13. Shimozawa, N., Ono, Y., Kimoto, S., et al. (2002) Abnormalities in cloned mice are not transmitted to the progeny. Genesis 34, 203–207.

    Article  PubMed  Google Scholar 

  14. Wells, D. N. (2003) Cloning in livestock agriculture. Reprod. Suppl. 61, 131–150.

    CAS  PubMed  Google Scholar 

  15. Wilson, J. M., Williams, J. D., Bondioli, K. R., Looney, C. R., Westhusin, M. E., and McCalla, D. F. (1995) Comparison of birth weight and growth characteristics of bovine calves produced by nuclear transfer (cloning), embryo transfer and natural mating. Anim. Reprod. Sci. 38, 73–83.

    Article  Google Scholar 

  16. Morrow, C. J. and Berg, M. C. (2003) Ultrasound guided transvaginal collection of amniotic and/or allantoic fluid from cattle. Theriogenology 59, 539.

    Google Scholar 

  17. Hill, J. R., Schlafer, D. H., Fisher, P. J., and Davies, C. J. (2002) Abnormal expression of trophoblast major histocompatibility complex class I antigens in cloned bovine pregnancies is associated with a pronounced endometrial lymphocytic response. Biol. Reprod. 67, 55–63.

    Article  CAS  PubMed  Google Scholar 

  18. Eigenmann, U. J. E., Schoon, H. A., Jahn, D., and Grunert, E. (1984) Neonatal respiratory distress syndrome in the calf. Vet. Rec. 114, 141–144.

    Article  CAS  PubMed  Google Scholar 

  19. Zaremba, W., Grunert, E., and Aurich, J. E. (1997) Prophylaxis of respiratory distress syndrome in premature calves by administration of dexamethasone or a prostaglandin F2α analogue to their dams before parturition. Am. J. Vet. Res. 58, 404–407.

    CAS  PubMed  Google Scholar 

  20. Renard, J. P., Chastant, S., Chesne, P., et al. (1999) Lymphoid hypoplasia and somatic cloning. Lancet 353, 1489–1491.

    Article  CAS  PubMed  Google Scholar 

  21. Lanza, R. P., Cibelli, J. B., Faber, D., et al. (2001) Cloned cattle can be healthy and normal. Science 294, 1893–1894.

    Article  CAS  PubMed  Google Scholar 

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© 2006 Humana Press Inc.

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Forsyth, J.T., Wells, D.N. (2006). Health and Neonatal Care of Bovine Clones. In: Verma, P.J., Trounson, A.O. (eds) Nuclear Transfer Protocols. Methods in Molecular Biology™, vol 348. Humana Press. https://doi.org/10.1007/978-1-59745-154-3_6

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  • DOI: https://doi.org/10.1007/978-1-59745-154-3_6

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-280-3

  • Online ISBN: 978-1-59745-154-3

  • eBook Packages: Springer Protocols

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