Abstract
Nuclear transfer (NT) using transgenic donor cells is an efficient means for generation of transgenic founder goats, especially in regard to the number of animals required to produce a transgenic founder expressing the protein of interest. Vectors can be designed for organ-specific expression and secretion of recombinant proteins within the target tissue. Furthermore, donor cells can be selected for gender, genetically modified to introduce the transgene of interest and screened for incorporation of the transgene into the genome before use in NT. This chapter describes methods for production of transgenic donor cells, subsequent NT embryo production, and transfer into recipients.
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Lazaris, A., Keyston, R., Karatzas, C.N., Keefer, C.L. (2006). Transgenesis Using Nuclear Transfer in Goats. In: Verma, P.J., Trounson, A.O. (eds) Nuclear Transfer Protocols. Methods in Molecular Biology™, vol 348. Humana Press. https://doi.org/10.1007/978-1-59745-154-3_14
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DOI: https://doi.org/10.1007/978-1-59745-154-3_14
Publisher Name: Humana Press
Print ISBN: 978-1-58829-280-3
Online ISBN: 978-1-59745-154-3
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