Summary
Isolation and characterization of neural stem cells and lineage-specific progenitors provide important information for central nervous system development study and regenerative medicine. We describe methods for dissection of rodent embryonic spinal cords by enzymatic separation, and isolation and enrichment (or purification) of neuronal and glial precursors at different developing stages by fluorescence-activated cell sorting.
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Acknowledgments
This work was supported by the University of Utah and Invitrogen. We thank Dr. J. Cai for critical reading of this manuscript. We thank all members of our laboratory for constant stimulating discussions. Several of the antibodies used were obtained from the Developmental Studies Hybridoma Bank (DSHB). DSHB is supported by a grant from National Institute of Neurological Disorders and Stroke. M.S.R. thanks Dr. Seema Rao for constant support through all phases of this project.
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© 2008 Humana Press, a part of Springer Science+Business Media, LLC
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Wu, Y., Liu, Y., Chesnut, J.D., Rao, M.S. (2008). Isolation of Neural Stem and Precursor Cells from Rodent Tissue. In: Weiner, L.P. (eds) Neural Stem Cells. Methods in Molecular Biology™, vol 438. Humana Press. https://doi.org/10.1007/978-1-59745-133-8_5
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DOI: https://doi.org/10.1007/978-1-59745-133-8_5
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