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Production and Characterization of Immortal Human Neural Stem Cell Line with Multipotent Differentiation Property

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Neural Stem Cells

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 438))

Summary

We document the protocols and methods for the production of immortalized cell lines of human neural stem cells from the human fetal central nervous system (CNS) cells by using a retroviral vector encoding v-myc oncogene. One of the human neural stem cell lines (HB1.F3) was found to express nestin and other specific markers for human neural stem cells, giving rise to three fundamental cell types of the CNS: neurons, astrocytes, and oligodendrocytes. After transplantation into the brain of mouse model of stroke, implanted human neural stem cells were observed to migrate extensively from the site of implantation into other anatomical sites and to differentiate into neurons and glial cells.

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Author information

Authors and Affiliations

  1. Division of Neurology, UBC Hospital, University of British Columbia, Vancouver, British Columbia

    Seung U. Kim MD,PhD, Atsushi Nagai MD, Eiji Nakagawa MD,PhD, Hyun B. Choi PhD, Jung H. Bang MSc & Hong J. Lee PhD

  2. Brain Disease Research Center, Ajou University School of Medicine, Suwon, Korea

    Seung U. Kim MD,PhD, Jung H. Bang MSc, Hong J. Lee PhD, Myung A. Lee PhD, Yong B. Lee PhD & In H. Park MD

Authors
  1. Seung U. Kim MD,PhD
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  2. Atsushi Nagai MD
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  3. Eiji Nakagawa MD,PhD
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  4. Hyun B. Choi PhD
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  5. Jung H. Bang MSc
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  6. Hong J. Lee PhD
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  7. Myung A. Lee PhD
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  8. Yong B. Lee PhD
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  9. In H. Park MD
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Editors and Affiliations

  1. Keck School of Medicine, University of Southern California, 2025 Zonal Ave., Los Angeles, CA 90033, USA

    Leslie P. Weiner

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© 2008 Humana Press, a part of Springer Science+Business Media, LLC

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Kim, S.U. et al. (2008). Production and Characterization of Immortal Human Neural Stem Cell Line with Multipotent Differentiation Property. In: Weiner, L.P. (eds) Neural Stem Cells. Methods in Molecular Biology™, vol 438. Humana Press. https://doi.org/10.1007/978-1-59745-133-8_10

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  • DOI: https://doi.org/10.1007/978-1-59745-133-8_10

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-846-1

  • Online ISBN: 978-1-59745-133-8

  • eBook Packages: Springer Protocols

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