Summary
The working definition of a stem cell includes self-renewal and the ability to differentiate into several cell types. There are also aspects of clonality and potency. Stem cells can be derived from early embryos after the formation of the blastocyst or from fetal, postnatal, or adult sources. Neural stem cells (NSCs) arise from embryonic ectoderm that forms neuroepithelial cells. The neuroepithelial cells generate radial glia that produce fetal and adult NSCs within the central nervous system (CNS). Adult NSC and restricted progenitors are found in the several regions of the CNS throughout life. Human embryonic stem cells, with their ability for self-renewal, clonal capacity, normal karyotype, and potential to form NSCs, easily may be the best source of NSCs and progenitors for treating disease. However, the complexity of NSCs, neural patterning, and the formation of multiple populations of neurons, astrocytes, and oligodendrocytes warrant the need for intense studies to characterize these cells and to define the microenvironment that will be needed to support them in the diseased CNS. Ways to produce well-defined populations, avoid oncogenicity, and ensure survival need to be clarified before clinical application can begin.
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References
Thomson, J. A., Itskovitz-Eldor, J., Shapiro, S. S., et al. (1998) Embryonic stem cell lines derived from human blastocysts. Science 282, 1145–1147.
Reubinoff, B. (2007) Current status of human embryonic stem cell research. Ethics Law Moral Philos. Reprod. Biomed. 2, 121–124.
Reynolds, B. A. and Weiss, S. (1992) Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system. Science 255, 1707–1710.
Lois, C. and Alvarez-Buylla, A. (1993) Proliferating subventricular zone cells in the adult mammalian forebrain can differentiate into neurons and glia. Proc. Natl. Acad. Sci. USA 90, 2074–2077.
Johe, K. K., Hazel, T. G., Muller, T., Dugich-Djordjevic, M. M., and McKay, R. D. (1996) Single factors direct the differentiation of stem cells from the fetal and adult central nervous system. Genes Dev. 10, 3129–3140.
Liu, Y. and Rao, M. S. (2003) Transdifferentiation: fact or artifact. J. Cell. Biochem. 88, 29–40.
Weissman, I. L., Anderson, D. J., and Gage, F. (2001) Stem and progenitor cells: origins, phenotypes, lineage commitments, and transdifferentiations. Annu. Rev. Cell Dev. Biol. 17, 387–403.
Rideout, W. M., 3rd, Eggan, K., and Jaenisch, R. (2001) Nuclear cloning and epigenetic reprogramming of the genome. Science 293, 1093–1098.
Weissman, I. L. (2000) Stem cells: units of development, units of regeneration, and units in evolution. Cell 100, 157–168.
Smith, A. G. (2001) Embryo-derived stem cells: of mice and men. Annu. Rev. Cell Dev. Biol. 17, 435–462.
Sasal, Y., Lu, B., Steinbelsser, H., and De Robertis, E. M. (1995) Regulation of neural induction by the Chd and Bmp-4 antagonistic patterning signals in Xenopus. Nature 378, 419.
Lamb, T. M., Knecht, A. K., Smith, W. C., et al. (1993) Neural induction by the secreted polypeptide noggin. Science 262, 713–718.
Oelgeschlager, M., Kuroda, H., Reversade, B., and De Robertis, E. M. (2003) Chordin is required for the Spemann organizer transplantation phenomenon in Xenopus embryos. Dev. Cell 4, 219–230.
Willert, K., Brown, J. D., Danenberg, E., et al. (2003) Wnt proteins are lipid-modified and can act as stem cell growth factors. Nature 423, 448–452.
Diez del Corral, R., Breitkreuz, D. N., and Storey, K. G. (2002) Onset of neuronal differentiation is regulated by paraxial mesoderm and requires attenuation of FGF signaling. Development 129, 1681–1691.
Mathis, L., Kulesa, P. M., and Fraser, S. E. (2001) FGF receptor signaling is required to maintain neural progenitors during Hensen’s node progression. Nat. Cell Biol. 3, 559–566.
Ericson, J., Muhr, J., Jessell. T. M., and Edlund, T. (1995) Sonic hedgehog: a common signal for ventral patterning along the rostrocaudal axis of the neural tube. Int. J. Dev. Biol. 39, 809–816.
Lee, S. K. and Pfaff, S. L. (2003) Synchronization of neurogenesis and motor neuron specification by direct coupling of bHLH and homeodomain transcription factors. Neuron 38, 731–745.
Sun, T., Echelard, Y., Lu, R., Yuk, D., Kaing, S., Stiles, C. D., and Rowitch, D. H. (2001) Olig bHLH proteins interact with homeodomain proteins to regulate cell fate acquisition in progenitors of the ventral neural tube. Curr. Biol. 11, 1413–1420.
Novitch, B. G., Chen, A. I., and Jessell, T. M. (2001) Coordinate regulation of motor neuron subtype identity and pan-neuronal properties by the bHLH repressor Olig2. Neuron 31, 773–789.
Zhou, Q., Choi, G., and Anderson, D. J. (2001) The bHLH transcription factor Olig2 promotes oligodendrocyte differentiation in collaboration with Nkx2.2. Neuron 31, 791–807.
Zhou, Q. and Anderson, D. J. (2002) The bHLH transcription factors OLIG2 and OLIG1 couple neuronal and glial subtype specification. Cell 109, 61–73.
Kriegstein, A. R. and Gotz, M. (2003) Radial glia diversity: a matter of cell fate. Glia 43, 37–43.
Malatesta, P., Hartfuss, E., and Gotz, M. (2000) Isolation of radial glial cells by fluorescent-activated cell sorting reveals a neuronal lineage. Development 127, 5253–5263.
Johansson, C. B. (2003) Mechanism of stem cells in the central nervous system. J. Cell. Physiol. 196, 409–418.
Gould, E., Vail, N., Wagers, M., and Gross, C. G. (2001) Adult-generated hippocampal and neocortical neurons in macaques have a transient existence. Proc. Natl. Acad. Sci. USA 98, 10910–10917.
Bernier, P. J., Bedard, A., Vinet, J., Levesque, M., and Parent, A. (2002) Newly generated neurons in the amygdala and adjoining cortex of adult primates. Proc. Natl. Acad. Sci. USA 99, 11464–11469.
Bedard, A., Cossette, M., Levesque, M., and Parent, A. (2002) Proliferating cells can differentiate into neurons in the striatum of normal adult monkey. Neurosci. Lett. 328, 213–216.
Bedard, A., Levesque, M., Bernier, P. J., and Parent, A. (2002) The rostral migratory stream in adult squirrel monkeys: contribution of new neurons to the olfactory tubercle and involvement of the antiapoptotic protein Bcl-2. Eur. J. Neurosci. 16, 1917–1924.
Zhao, M., Momma, S., Delfani, K., et al. (2003) Evidence for neurogenesis in the adult mammalian substantia nigra. Proc. Natl. Acad. Sci. USA 100, 7925–7930.
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© 2008 Humana Press, a part of Springer Science+Business Media, LLC
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Weiner, L.P. (2008). Definitions and Criteria for Stem Cells. In: Weiner, L.P. (eds) Neural Stem Cells. Methods in Molecular Biology™, vol 438. Humana Press. https://doi.org/10.1007/978-1-59745-133-8_1
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DOI: https://doi.org/10.1007/978-1-59745-133-8_1
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