Abstract
Regulatory machinery for gene expression, replication, and repair are architecturally organized in nuclear microenvironments. This compartmentalization provides threshold concentrations of macromolecules for the organization and assembly of regulatory complexes for combinatorial control. A mechanistic under standing of biological control requires the combined application of molecular, cellular, biochemical, and in vivo genetic approaches. This chapter provides methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.
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Acknowledgments
Studies reported in this article were in part supported by grants from NIH (5PO1CA82834-05, 5PO1AR048818-05, 2R01GM32010, 5R01AR049069). The authors thank Judy Rask for editorial assistance with preparation of the manuscript.
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© 2008 Humana Press, a part of Springer Science+Business Media, LLC
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Pockwinse, S.M. et al. (2008). In Situ Nuclear Organization of Regulatory Machinery. In: Westendorf, J.J. (eds) Osteoporosis. Methods In Molecular Biology™, vol 455. Humana Press. https://doi.org/10.1007/978-1-59745-104-8_17
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DOI: https://doi.org/10.1007/978-1-59745-104-8_17
Publisher Name: Humana Press
Print ISBN: 978-1-58829-828-7
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