Multiple Roles of Viral Replication Proteins in Plant RNA Virus Replication

  • Peter  D. Nagy
  • Judit Pogany
Part of the Methods in Molecular Biology™ book series (MIMB, volume 451)

Abstract

Identification of the roles of replication factors represents one of the major frontiers in current virus research. Among plant viruses, the positive-stranded (+) RNA viruses are the largest group and the most widespread. The central step in the infection cycles of (+) RNA viruses is RNA replication, which leads to rapid production of huge number of viral (+) RNA progeny in the infected plant cells. The RNA replication process is carried out by the virus-specific replicase complex consisting of viral RNA-dependent RNA polymerase, one or more auxiliary viral replication proteins, and host factors, which assemble in specialized membranous compartments in infected cells. Replication is followed by cell-to-cell and long- distance movement to invade the entire plant and/or encapsidation to facilitate transmission to new plants. This chapter provides an overview of our current understanding of the role of viral replication proteins during genome replication. The recent significant progress in this research area is based on development of powerful in vivo and in vitro approaches, including replicase assays, reverse genetic approaches, intracelular localization studies and the use of plant or yeast model hosts.

Keywords

Brome mosaic virus Tomato bushy stunt virus Tobacco mosaic virus RNA-dependent RNA polymerase replicase helicase chaperone 

Notes

Acknowledgments

The authors thank Drs. H. Jaag, Z. Li, and R. Wang for discussion. The authors apologize to those colleagues whose works on replication of (+)RNA viruses were not mentioned in this review due to page restrictions. This work was supported by NIH-NIAID.

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Copyright information

© Humana Press, a part of Springer Science + Business Media, LLC 2008

Authors and Affiliations

  • Peter  D. Nagy
    • 1
  • Judit Pogany
    • 1
  1. 1.Department of Plant PathologyUniversity of KentuckyLexingtonUSA

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