Abstract
Posttranslational modification of proteins is a key regulatory mechanism in a variety of cellular processes. This chapter outlines the concepts and methods used to investigate protein phosphorylation and its physiological relevance during plant virus infection. Rather than providing an exhaustive review of the experimental protocols for protein phosphorylation analysis, we focus on methods that can be used to study phosphorylation of viral proteins. We address the following points: how to determine that a viral protein of interest is phosphorylated; how to map the phosphorylation sites; how to identify the protein kinase(s) involved. Finally, we describe a number of useful strategies to evaluate the biological significance of phosphorylation.
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Acknowledgments
The authors thank Dr. Pietri Puustinen for help with writing the immunostaining protocol, Dr. Leena Valmu and Mr. Alun Parsons for critical reading of the manuscript. This work was supported by the Academy of Finland (grant 206870).
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© 2008 Humana Press, a part of Springer Science + Business Media, LLC
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Mäkinen, K.M., Ivanov, K.I. (2008). Phosphorylation Analysis of Plant Viral Proteins. In: Foster, G.D., Johansen, I.E., Hong, Y., Nagy, P.D. (eds) Plant Virology Protocols. Methods in Molecular Biology™, vol 451. Humana Press. https://doi.org/10.1007/978-1-59745-102-4_24
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DOI: https://doi.org/10.1007/978-1-59745-102-4_24
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