Abstract
The ability to combine nucleic acid hybridisation or immunospecific reactions with structural and ultrastructural analysis of virus-infected tissues has provided the opportunity to resolve the spatial details of infection with respect to the production of virus-specific products and the nature of the host response. These technologies may seem lengthy and complex but offer high rewards in terms of revealing the details of host—virus interactions not otherwise accessible.
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References
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Acknowledgments
Kim Findlay is acknowledged for advice on the methodology for in situ hybridisation for EM. Z. H. was supported by Hungarian Scientific Research Fund (OTKA; K61461) and Bolyai Janos Fellowship. The John Innes Centre is grant-aided by the United Kingdom Biotechnology and Biological Research Council.
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© 2008 Humana Press, a part of Springer Science + Business Media, LLC
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Maule, A.J., Havelda, Z. (2008). In Situ Detection of Plant Viruses and Virus-Specific Products. In: Foster, G.D., Johansen, I.E., Hong, Y., Nagy, P.D. (eds) Plant Virology Protocols. Methods in Molecular Biology™, vol 451. Humana Press. https://doi.org/10.1007/978-1-59745-102-4_14
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DOI: https://doi.org/10.1007/978-1-59745-102-4_14
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