Begomoviruses: Molecular Cloning and Identification of Replication Origin

  • Lilian H. Florentino
  • Anésia A. Santos
  • Francisco M. Zerbini
  • Elizabeth P. B. Fontes
Part of the Methods in Molecular Biology™ book series (MIMB, volume 451)


The Begomovirus genus is the largest genus of the Geminiviridae family and comprises the whitefly transmitted geminiviruses that infect dicotyledonous plants. They can be either mono or bipartite. In this chapter, we describe the cloning of begomovirus replication modules and the subsequent functional characterization of geminivirus replication origins.


Geminivirus Begomovirus Origin of replication Replication assay Replicon module 


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Copyright information

© Humana Press, a part of Springer Science + Business Media, LLC 2008

Authors and Affiliations

  • Lilian H. Florentino
    • 1
  • Anésia A. Santos
    • 1
  • Francisco M. Zerbini
    • 2
  • Elizabeth P. B. Fontes
    • 1
  1. 1.Departamento de Bioquímica e Biologia MolecularBIOAGRO-UniversidadeViçosaBrazil
  2. 2.Departamento de BIOAGROFitopatologia UniversidadeViçosaBrazil

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