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Plant Virology Protocols pp 145–166Cite as

Begomoviruses: Molecular Cloning and Identification of Replication Origin

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 451))

Abstract

The Begomovirus genus is the largest genus of the Geminiviridae family and comprises the whitefly transmitted geminiviruses that infect dicotyledonous plants. They can be either mono or bipartite. In this chapter, we describe the cloning of begomovirus replication modules and the subsequent functional characterization of geminivirus replication origins.

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References

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Author information

Authors and Affiliations

  1. Departamento de Bioquímica e Biologia Molecular, BIOAGRO-Universidade, Federal de Viçosa-36571.000, Viçosa, MG, Brazil

    Lilian H. Florentino, Anésia A. Santos & Elizabeth P. B. Fontes

  2. Departamento de BIOAGRO, Fitopatologia Universidade, Federal de Viçosa-36571.000, Viçosa, MG, Brazil

    Francisco M. Zerbini

Authors
  1. Lilian H. Florentino
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  2. Anésia A. Santos
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  3. Francisco M. Zerbini
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  4. Elizabeth P. B. Fontes
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Editor information

Editors and Affiliations

  1. University of Bristol, Bristol, UK

    Gary D. Foster

  2. University of Aarhus, Aarhus, Denmark

    I. Elisabeth Johansen

  3. University of Warwick, Warwick, UK

    Yiguo Hong

  4. University of Kentucky, Kentucky, USA

    Peter D. Nagy

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© 2008 Humana Press, a part of Springer Science + Business Media, LLC

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Florentino, L.H., Santos, A.A., Zerbini, F.M., Fontes, E.P.B. (2008). Begomoviruses: Molecular Cloning and Identification of Replication Origin. In: Foster, G.D., Johansen, I.E., Hong, Y., Nagy, P.D. (eds) Plant Virology Protocols. Methods in Molecular Biology™, vol 451. Humana Press. https://doi.org/10.1007/978-1-59745-102-4_11

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  • DOI: https://doi.org/10.1007/978-1-59745-102-4_11

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-827-0

  • Online ISBN: 978-1-59745-102-4

  • eBook Packages: Springer Protocols

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