Analysis of Geminivirus DNA Replication by 2-D Gel
The technique described was developed for the separation of begomovirus DNA. DNA products resulting from and during geminiviral replication are characterized by the application of strand-specific separation and identification by strand-specific DNA probing of Southern blots. The mapping of the initiation site of complementary-strand DNA synthesis, by this technique is also presented.
KeywordsGeminivirus ssDNA dsDNA Rolling circle replication