Analysis of Geminivirus DNA Replication by 2-D Gel

  • Keith Saunders
Part of the Methods in Molecular Biology™ book series (MIMB, volume 451)


The technique described was developed for the separation of begomovirus DNA. DNA products resulting from and during geminiviral replication are characterized by the application of strand-specific separation and identification by strand-specific DNA probing of Southern blots. The mapping of the initiation site of complementary-strand DNA synthesis, by this technique is also presented.


Geminivirus ssDNA dsDNA Rolling circle replication 


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Copyright information

© Humana Press, a part of Springer Science + Business Media, LLC 2008

Authors and Affiliations

  • Keith Saunders
    • 1
  1. 1.Biological ChemistryJohn Innes CentreNorwichUK

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