Summary
Recent studies have shown that cell-free fetal DNA in maternal plasma can be enriched by means of size fractionation. This technique makes use of the smaller size of fetal DNA fragments compared with maternal DNA fragments isolated simultaneously. On this basis, a highly improved detection of fetal single gene point mutations is permitted. Here, we introduce the use of agarose gel electrophoresis for the size fractionation of cell-free DNA from maternal plasma and the detection of fetal β-thalassemia mutations in the size-fractionated cell-free DNA by using peptide nucleic acid-clamping polymerase chain reaction (PCR) combined with an allele-specific real-time PCR assay. Matrix-assisted laser desorption ionization/time of flight mass spectrometry has been reliably used for detection of fetal single gene point mutations in maternal plasma. We also present its use for genotyping paternally inherited single-nucleotide polymorphism alleles in the size-fractionated cell-free DNA from maternal plasma.
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We thank Dr. D. J. Huang for helpful comments and proofreading.
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© 2008 Humana Press, a part of Springer Science+Business Media, LLC
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Li, Y., Holzgreve, W., Hahn, S. (2008). Size Fractionation of Cell-Free DNA in Maternal Plasma and Its Application in Noninvasive Detection of Fetal Single Gene Point Mutations. In: Hahn, S., Jackson, L.G. (eds) Prenatal Diagnosis. Methods in Molecular Biology™, vol 444. Humana Press. https://doi.org/10.1007/978-1-59745-066-9_19
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DOI: https://doi.org/10.1007/978-1-59745-066-9_19
Publisher Name: Humana Press
Print ISBN: 978-1-58829-803-4
Online ISBN: 978-1-59745-066-9
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