Summary
Immunofluorescence microscopy is a very sensitive serological test which harnesses both the power of antibodies to bind to targets along with the use of the flourescence microscope to visualise the structures to which they bind. Antibody binding is visualised by the fluorescent emission from a marker molecule bound to the antibody. The technique is of particular use in bacteriology and pathology where the location and morphology of the bacterial cells can be viewed along with the location of the fluorescently labelled antibodies.
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The authors want to thank the contributors of the Annex II as published in Anonymous 2006 to use its information.
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Janse, J.D., Kokoskova, B. (2009). Indirect Immunofluorescence Microscopy for the Detection and Identification of Plant Pathogenic Bacteria (In Particular for Ralstonia solanacearum). In: Burns, R. (eds) Plant Pathology. Methods in Molecular Biology, vol 508. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-062-1_8
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DOI: https://doi.org/10.1007/978-1-59745-062-1_8
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