Summary
A range of immunisation techniques can be used for the successful production of antibodies. The choice of method used is dependent on the nature of the antigen and the type of antibody required by the user.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Roitt, I., Brostoff, J. and Male, D. (1996) Immunology. Mosby, London, 8.13–8.16.
Kennet, R. H., Denis, K. A., Tung, A. S. and Klinman, N. R. (1978) Hybrid plasmocytoma production: fusions with adult spleen cells, monoclonal spleen fragments, neonatal spleen cells and human spleen cells. Current Topics in Microbiological Immunology 81, 495.
Hsu, H. T., Lee, I., Davis, R. E. and Wang, Y. C. (1990) Immunisation for generation of hybridoma antibodies specifically reacting with plants infected with a mycoplasma like organism (MLO) and their use in detection of MLO antigens. Phytopathology 80, 946–950.
Hsu, H. T., Wang, Y. C., Lawson, R. H., Wang, M. and Gonzalves, D. (1990) Spenocytes of mice with induced immunological tolerance to plant antigens for construction of hybridomas secreting tomato spotted wilt virus-specific antibodies Phytopathology 80, 158–162.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2009 Humana Press, a part of Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Burns, R. (2009). Immunisation strategies for Antibody Production. In: Burns, R. (eds) Plant Pathology. Methods in Molecular Biology, vol 508. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-062-1_3
Download citation
DOI: https://doi.org/10.1007/978-1-59745-062-1_3
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-58829-799-0
Online ISBN: 978-1-59745-062-1
eBook Packages: Springer Protocols