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Development of Enzyme Linked, Tissue Blot and Dot Blot Immunoassays for Plant Virus Detection

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Plant Pathology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 508))

Summary

Immunoassays are among the most powerful and useful techniques for analysis of biological materials. There are numerous variations in which immunoassays can be performed. Coupled with enzyme, using chromogenic substrates, the enzyme immunoassay technique is used to trace the target antigen in tissues. The technique also is used to measure the concentration of antigen in tissue extracts. This chapter provides fundamental information that is needed to carry out the routinely used procedures in plant virus research.

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References

  1. Goding, J.W. (1978). Use of staphylococcal protein A as an immunological reagent. J. Immunol. Methods 20:241–253.

    Article  PubMed  CAS  Google Scholar 

  2. Langone, J.J. (1982). Protein A of Staphylococcus aureus and related immunoglobulin receptors produced by streptococci and pneumonococci. Adv. Immunol. 32:157–251.

    Article  PubMed  CAS  Google Scholar 

  3. Guesdon, J.L., Ternynck, T., and Avrameas, S. (1979). The use of avidin–biotin interaction of immunoenzymatic techniques. J. Histochem. Cytochem. 27:1131–1139.

    Article  PubMed  CAS  Google Scholar 

  4. Clark, M.F., and Adam, A.N. (1977). Characterization of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J. Gen. Virol. 34:475–483.

    Article  PubMed  CAS  Google Scholar 

  5. Lin, N.S., Hsu, Y.H., and Hsu, H.T. (1990). Immunological detection of plant viruses and a mycoplasma like organism by direct tissue blotting on nitrocellulose membranes. Phytopathlogy 80:824–828.

    Article  CAS  Google Scholar 

  6. Hsu, H.T., Lee, I.M., Davis, R.E., and Wang, Y.C. (1990). Immunization for generation of hybridoma antibodies specifically reacting with plants infected with a mycoplasma like organism (MLO) and their use in detection of MLO antigens. Phytopathology 80:946–950.

    Article  CAS  Google Scholar 

  7. Avrameas, S. (1969). Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies. Immunochemistry 6:43–52.

    Article  PubMed  CAS  Google Scholar 

  8. Gershoni, J.M., and Palade, G.E. (1982). Electrophoretic transfer of proteins from sodium dodecyl sulphate–polyacrylamide gels to a positively charged membrane filter. Anal. Biochem. 12: 396–405.

    Article  Google Scholar 

  9. Makkouk, K.M., Hsu, H.T., and Kumari, S.G. (1993). Detection of three plant viruses by dot-blot and tissue-blot immunoassays using chemiluminescent and chromogenic substrates. J. Phytopathol. 139:97–102.

    Article  CAS  Google Scholar 

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Correspondence to Hei-ti Hsu .

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Hsu, Ht. (2009). Development of Enzyme Linked, Tissue Blot and Dot Blot Immunoassays for Plant Virus Detection. In: Burns, R. (eds) Plant Pathology. Methods in Molecular Biology, vol 508. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-062-1_2

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  • DOI: https://doi.org/10.1007/978-1-59745-062-1_2

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-58829-799-0

  • Online ISBN: 978-1-59745-062-1

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