Isolation and Manipulation of Mammalian Neural Stem Cells In Vitro

  • Claudio GiachinoEmail author
  • Onur Basak
  • Verdon Taylor
Part of the Methods in Molecular Biology book series (MIMB, volume 482)


Neural stem cells are potentially a source of cells not only for replacement therapy but also as drug vectors, bringing bioactive molecules into the brain. Stem cell-like cells can be isolated readily from the human brain, thus, it is important to find culture systems that enable expansion in a multipotent state to generate cells that are of potential use for therapy. Currently, two systems have been described for the maintenance and expansion of multipotent progenitors, an adhesive substrate bound and the neurosphere culture. Both systems have pros and cons, but the neurosphere may be able to simulate the three-dimensional environment of the niche in which the cells reside in vivo. Thus, the neurosphere, when used and cultured appropriately, can expand and provide important information about the mechanisms that potentially control neural stem cells in vivo.

Key words

Neural stem cells embryonic neurogenesis adult neurogenesis Notch signaling neurosphere differentiation 


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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  1. 1.Department of Molecular EmbryologyMax Planck Institute of ImmunobiologyGermany

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