Investigating the Interactions Between Haemopoietic Stem Cells and Their Niche: Methods for the Analysis of Stem Cell Homing and Distribution Within the Marrow Following Transplantation

  • Brenda Williams
  • Susan K. Nilsson
Part of the Methods in Molecular Biology book series (MIMB, volume 482)


Interactions between haemopoietic stem cells (HSC) and their microenvironment serve multiple functions including the attraction to and retention and regulation in the bone marrow HSC niche. However, the cell adhesion molecules involved, their HSC receptors and the mechanisms underpinning these processes remain poorly understood. An ability to thoroughly investigate the roles of specific molecules in this process relies on a variety of in vitro and in vivo assays including the assessment of a HSC ability to home to the bone marrow and analysis of its lodgement within the bone marrow.

Key words

Niche haemopoietic stem cells bone marrow homing 


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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Brenda Williams
    • 1
  • Susan K. Nilsson
    • 1
  1. 1.Australian Stem Cell Centre, Monash UniversityAustralia

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