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Derivation of Contractile Smooth Muscle Cells from Embryonic Stem Cells

  • Sanjay Sinha
  • Mark H. Hoofnagle
  • Gary K. Owens
Part of the Methods in Molecular Biology book series (MIMB, volume 482)

Abstract

Smooth muscle cells (SMCs) play a key role in vascular physiology and pathology. An appreciation of normal SMCs developmental mechanisms will likely lead to a better understanding of disease processes and potentially to novel treatment strategies. We present a method for generating relatively pure populations of SMCs from embryonic stem cells (ESC) which display appropriate excitation and contractile responses to vasoactive agonists. We also present protocols for assessment of SMCs purity and identity by immunofluorescence, quantitative RT-PCR, and FACS. This ESC-based system has tremendous potential for studying developmental regulation of SMC lineage, as well as for possible SMC tissue engineering.

Key words

Stem cell smooth muscle embryoid body contraction 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Sanjay Sinha
    • 1
  • Mark H. Hoofnagle
    • 2
  • Gary K. Owens
    • 2
  1. 1.Division of Cardiovascular MedicineUniversity of Cambridge, Addenbrooke’s HospitalUK
  2. 2.Department of Molecular Physiology and Biological PhysicsCardiovascular Research Center, The University of VirginiaCharlottesvilleUSA

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