Prospective Isolation of Mesenchymal Stem Cells from Mouse Compact Bone

  • Brenton J. Short
  • Nathalie Brouard
  • Paul J. Simmons
Part of the Methods in Molecular Biology book series (MIMB, volume 482)


Bone marrow from numerous species, including rodents and man, has been shown to contain a rare population of cells known as marrow stromal cells or mesenchymal stem cells (MSC). Given the innate ability of these cells to give rise to multiple tissue types including bone, fat and cartilage, there is considerable interest in utilizing MSC in a broad repertoire of cell-based therapies for the treatment of human disease. In order for such therapies to be realized, a preclinical animal model in which to refine strategies utilizing MSC is required.

We have described methodology allowing for the prospective isolation by fluorescence activated cell sorting (FACS) of a highly purified population of MSC from murine compact bone (CB). These cells are multipotent and capable of extensive proliferation in vitro and thus represent an ideal source of cells with which to explore both the fundamental biology of MSC and their efficacy in a variety of cellular therapies.

Key words

Mesenchymal Stem Cell MSC CFU-F multipotent FACS compact bone 


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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Brenton J. Short
    • 1
  • Nathalie Brouard
    • 1
  • Paul J. Simmons
    • 1
  1. 1.Peter MacCallum Cancer CentreAustralia

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