Isolation, Expansion, and Differentiation of Mouse Skin-Derived Precursors

  • Karl J.L. Fernandes
  • Freda D. Miller
Part of the Methods in Molecular Biology book series (MIMB, volume 482)


The isolation and experimental manipulation of multipotent precursors is of increasing therapeutic relevance. We recently reported the generation of cultures of Skin-derived Precursors (‘SKPs’), multipotent cells that can be isolated from the dermis of embryonic, neonatal, and adult rodent skin (1), and from adult human skin (2) SKPs have similarities to stem cells of the embryonic neural crest (3), and differentiate into a variety of neural and mesodermal cell phenotypes, including peripheral neurons and glial cells, smooth muscle cells, bone, cartilage, and adipocytes (35). Here, we detail the establishment, propagation, neural differentiation, and immunocytochemical analysis of SKP cultures.

Key words

Neural crest stem cells SKPs NCSCs neurospheres neurogenic potential 


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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Karl J.L. Fernandes
    • 1
  • Freda D. Miller
    • 2
  1. 1.Département de pathologie et biologie cellulaireUniversité de MontréalMontréalCanada
  2. 2.Programs in Developmental Biology and Brain and Behaviour, Toronto Hospital for Sick Children, and Departments of Molecular & Medical Genetics and PhysiologyUniversity of TorontoTorontoCanada

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