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Derivation and Manipulation of Murine Embryonic Stem Cells

  • Alexander Meissner
  • Sarah Eminli
  • Rudolf Jaenisch
Part of the Methods in Molecular Biology book series (MIMB, volume 482)

Abstract

Pluripotent embryonic stem (ES) cell lines were first isolated over 25 years ago and remain an essential tool in molecular and developmental biology to this day. In particular, the use of homologous recombination and subsequent generation of ES-derived mice has greatly facilitated research across all fields. Moreover, ES cells represent an extremely attractive model to study events in early development. In this chapter, we will describe the derivation and propagation of murine ES cells. This is followed by a description of targeting ES cells and a protocol for the generation of mice by diploid and tetraploid blastocyst injections.

Key words

Embryonic stem cells pluripotency homologous recombination 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Alexander Meissner
    • 1
  • Sarah Eminli
    • 2
  • Rudolf Jaenisch
    • 3
  1. 1.Whitehead Institute for Biomedical ResearchMassachusetts Institute of TechnologyCambridgeUSA
  2. 2.Center for Regenerative Medicine and Cancer Center, Massachusetts General HospitalHarvard Medical School and Harvard Stem Cell InstituteBostonUSA
  3. 3.Whitehead Institute for Biomedical ResearchMassachusetts Institute of TechnologyCambridgeUSA

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