Summary
Genome-wide transcriptional profiling provides a rich source of data for the validation and annotation of organelle proteomic data. Organelle biogenesis is in most cases accompanied by upregulation of genes encoding organelle-specific proteins. Consequently, identification of genes whose expression correlates with organelle assembly leads to a candidate list that can be cross-checked with a preliminary organelle proteome. When proteins are found in the proteome and the corresponding genes are found to have organelle assembly-correlated expression, this greatly increases our confidence that those proteins are true components of the organelle and not contamination. Such an approach can be used to narrow down a preliminary proteomic data set and help us to focus on a smaller sub-set of proteins that are supported by transcriptomic cross-validation.
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Marshall, W.F. (2008). Use of Transcriptomic Data to Support Organelle Proteomic Analysis. In: Pflieger, D., Rossier, J. (eds) Organelle Proteomics. Methods in Molecular Biology™, vol 432. Humana Press. https://doi.org/10.1007/978-1-59745-028-7_27
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DOI: https://doi.org/10.1007/978-1-59745-028-7_27
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