Summary
Chromosomes are supermolecules that contain most of the DNA within a cell and are visible under optical and electron microscopes. Although they were observed at the earliest stage of genetics, their fundamental structure is not yet understood. The reasons for this are debated among researchers; however, it is clear that the accumulation of metaphase chromosomes for their biochemical analysis has been a significant challenge. In this chapter, a method is described for accumulating and preparing human metaphase chromosomes in sufficient amount to perform their proteome analysis. Preparation and separation methods of chromosome proteins are described, followed by a protocol for their identification by mass spectrometry.
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Acknowledgements
The authors thank Profs Schihiro Matsunaga, and Shin’ichiro Kajiyama for their collaboration during the research on chromosome proteomics. This work was supported by Special Coordination Funds from the Ministry of Education, Culture, Sports, Science and Technology, Japan to K. F.
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© 2008 Humana Press, a part of Springer Science+Business Media, LLC
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Fukui, K., Takata, H., Uchiyama, S. (2008). Preparation Methods of Human Metaphase Chromosomes for their Proteome Analysis. In: Pflieger, D., Rossier, J. (eds) Organelle Proteomics. Methods in Molecular Biology™, vol 432. Humana Press. https://doi.org/10.1007/978-1-59745-028-7_10
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DOI: https://doi.org/10.1007/978-1-59745-028-7_10
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