Abstract
The National Institutes of Health Xenopus Initiative is a concerted effort to interact with the Xenopus research community to identify the community’s needs; to devise strategies to meet those needs; and to support, oversee, and coordinate the resulting projects. This chapter provides a brief description of several genetic and genomic resources generated by this initiative and explains how to access them. The resources described in this chapter are (1) complementary deoxyribonucleic acid (cDNA) libraries and expressed sequence tag (EST) sequences; (2) UniGene clusters; (3) full-insert cDNA sequences; (4) a genetic map; (5) genomic libraries; (6) a physical map; (7) genome sequence; (8) microarrays; (9) mutagenesis and phenotyping; and (10) bioinformatics. The descriptions presented here were based on data that were available at the time of manuscript submission. Because these are ongoing projects, they are constantly generating new data and analyses. The Web sites cited in each subheading present current data and analyses.
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References
Klein, S. L., Strausberg, R. L., Wagner, L., Pontius, J., Clifton, S. W., and Richardson, P. (2002) Genetic and genomic tools for Xenopus research: the NIH Xenopus initiative. Dev. Dyn. 225, 384–391.
Gerhard, D. S., Wagner, L., Feingold, E. A., et al. (2004) The status, quality and expansion of the NIH full-length cDNA project (MGC). Genome Res. 14, 2121–2127.
Stanford, I. S., Lieberman, S. L., Wong, V. L., and Ruderman, J. D. (2003) Regulation of the G2/M transition in oocytes of Xenopus tropicalis. Dev. Biol. 260, 438–448.
Toth, G., Gaspari, Z., and Jurka I. (2000) Microsatellites in different eukaryotic genomes: survey and analysis. Genome Res. 10, 967–981.
McPherson, J. D., Marra, M., Hillier, L., et al. (2001) A physical map of the human genome. Nature 409, 934–941.
Gregory, S. G., Sekhon, M., Schein, J., et al. (2002) A physical map of the mouse genome. Nature 418, 743–750.
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© 2006 Humana Press Inc., Totowa, NJ
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Klein, S.L. et al. (2006). Resources for Genetic and Genomic Studies of Xenopus . In: Liu, X.J. (eds) Xenopus Protocols. Methods in Molecular Biology™, vol 322. Humana Press. https://doi.org/10.1007/978-1-59745-000-3_1
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DOI: https://doi.org/10.1007/978-1-59745-000-3_1
Publisher Name: Humana Press
Print ISBN: 978-1-58829-362-6
Online ISBN: 978-1-59745-000-3
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