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Three-Dimensional Reconstruction of Single Particles in Electron Microscopy

Image Processing

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Cell Imaging Techniques

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 319))

Abstract

Three-dimensional electron microscopy of single macromolecular assemblies has made large strides forward over the last decade. A large number of image processing techniques have been developed and many have found general distribution. For the proper usage of the wide range of available techniques, a clear concept of all processing steps is essential. This chapter provides step-by-step instruction for the three-dimensional reconstruction of an unknown macromolecule. Where possible, the limitations of the techniques are explained. The chapter attempts to be sufficiently general such so as not to adhere to a single image processing system. Described are alignment techniques for two and three dimensions, classification procedures, and the usage of three-dimensional reconstruction algorithms.

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Authors and Affiliations

  1. Department of Molecular Physiology and Biophysics, University of Vermont, Burlington, VT

    Michael Radermacher & Teresa Ruiz

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  1. Michael Radermacher
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  2. Teresa Ruiz
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Editor information

Editors and Affiliations

  1. Department of Pathology, University of Vermont, Burlington, VT

    Douglas J. Taatjes  & Brooke T. Mossman  & 

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© 2006 Humana Press Inc.

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Radermacher, M., Ruiz, T. (2006). Three-Dimensional Reconstruction of Single Particles in Electron Microscopy. In: Taatjes, D.J., Mossman, B.T. (eds) Cell Imaging Techniques. Methods in Molecular Biology™, vol 319. Humana Press. https://doi.org/10.1007/978-1-59259-993-6_20

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  • DOI: https://doi.org/10.1007/978-1-59259-993-6_20

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-157-8

  • Online ISBN: 978-1-59259-993-6

  • eBook Packages: Springer Protocols

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