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Secretory Vesicle Swelling by Atomic Force Microscopy

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Cell Imaging Techniques

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 319))

Abstract

The swelling of secretory vesicles has been implicated in exocytosis, but the underlying mechanism of vesicle swelling remained unknown. Earlier studies from our laboratory demonstrated the association of the α-subunit of heterotrimeric GTP-binding protein G±i3 with zymogen granule membrane and implicated its involvement in vesicle swelling. Mas7, an active mastoparan analog known to stimulate Gi proteins, was found to stimulate the GTPase activity of isolated zymogen granules and cause swelling. Increase in vesicle size in the presence of GTP, NaF, and Mas7 were irreversible and found to be KCl sensitive. However, Ca2+ had no effect on zymogen granule size. Taken together, these results indicated that zymogen granules, the membrane-bound secretory vesicles in exocrine pancreas, swell in response to GTP mediated by a G±i3 protein. Subsequently, our studies demonstrated that the water channel aquaporin-1 (AQP1) is also present at the zymogen granule membrane and participates in rapid GTP-induced and G±i3-mediated vesicular water gating and swelling. Isolated zymogen granules exhibit low basal water permeability. However, exposure of granules to GTP results in a marked potentiation of water entry. Treatment of zymogen granules with the known water channel inhibitor Hg2+ is accompanied by a reversible loss in both the basal and GTP-stimulable water entry and vesicle swelling. Introduction of AQP1-specific antibody raised against the carboxy-terminal domain of AQP1 blocked GTP-stimulable swelling of vesicles. Our results demonstrate that AQP1 associated at the zymogen granule membrane is involved in basal GTP-induced and G±i3-mediated rapid gating of water into zymogen granules of the exocrine pancreas.

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Author information

Authors and Affiliations

  1. Department of Physiology, Wayne State University School of Medicine, Detroit, MI

    Sang-Joon Cho & Bhanu P. Jena

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  1. Sang-Joon Cho
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  2. Bhanu P. Jena
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Editor information

Editors and Affiliations

  1. Department of Pathology, University of Vermont, Burlington, VT

    Douglas J. Taatjes  & Brooke T. Mossman  & 

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© 2006 Humana Press Inc.

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Cho, SJ., Jena, B.P. (2006). Secretory Vesicle Swelling by Atomic Force Microscopy. In: Taatjes, D.J., Mossman, B.T. (eds) Cell Imaging Techniques. Methods in Molecular Biology™, vol 319. Humana Press. https://doi.org/10.1007/978-1-59259-993-6_16

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  • DOI: https://doi.org/10.1007/978-1-59259-993-6_16

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-157-8

  • Online ISBN: 978-1-59259-993-6

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