Abstract
Affinity chromatography is ideally suited to the purification of pharmaceutical proteins due to its unique bio-specificity characteristics. Tailor-made affinity ligands that represent a promising class of synthetic affinity ligands have been developed to target specific proteins and designed to mimic peptidal templates, natural biological recognition motifs, or complementary surface-exposed residues. These biomimetic ligands have been generated by a combination of rational design, combinatorial library synthesis, and subsequent screening of potential leads against target proteins. Small ligands based on a triazine scaffold also present exceptional selectivity and stability, which allows their use in harsh manufacturing environments.
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References
Lowe, C. R. (2001) Combinatorial approaches to affinity chromatography. Curr. Opin. Chem. Biol. 5, 248–256.
Lowe, C. R., Burton, S. J., Burton, N. P., Alderton, W. K., Pitts, J. M., and Thomas, J. A. (1992) Designer dyes-biomimetic ligands for the purification of pharmaceutical proteins by affinity-chromatography. Trends Biotechnol. 10, 442–448.
Ansell, R. J. and Lowe, C. R. (1999) Artificial redox coenzymes: biomimetic analogues of NAD(+). Appl. Microbiol. Biotechnol. 51, 703–710.
Li, R. X., Dowd, V., Stewart, D. J., Burton, S. J., and Lowe, C. R. (1998) Design, synthesis, and application of a Protein A mimetic. Nat. Biotechnol. 16, 190–195.
Snyder, S. L. and Sobocinski, P. Z. (1975) An improved 2,4,6-trinitrobenzene sulfonic acid method for the determination of amines. Anal. Biochem. 64, 284–288.
Kaiser, E., Colescott, R., Bossinger, C. D., and Cook, P. I. (1970) Color test for detection of free terminal amino groups in the solid-phase synthesis of peptides. Anal. Biochem. 34, 595–598.
Morrill, P. R., Gupta, G., Sproule, K., et al. (2002) Rational combinatorial chemistry-based selection, synthesis and evaluation of an affinity adsorbent for recombinant human clotting factor VII. J. Chromatogr. B 774, 1–15.
Palanisamy, U. D., Hussain, A., Iqbal, S., Sproule, K., and Lowe, C. R. (1999) Design, synthesis and characterisation of affinity ligands for glycoproteins. J. Mol. Recognit. 12, 57–66.
Burton, N. P. (1992) Design of novel affinity adsorbents for the purification of trypsin-like proteases. J. Mol. Recognit. 5, 55–68.
Filippusson, H., Erlendsson, L. S., and Lowe, C. R. (2000) Design, synthesis and evaluation of biomimetic affinity ligands for elastases. J. Mol. Recognit. 13, 370–381.
Lowe, C. R., Roque, A. C. A., and Taipa, M. A. (2003) Affinity Adsorbents for Immunoglobulins PCT/GB 03/04524.
Roque, A. C. A., Taipa, M. A., and Lowe, C. R. (2004) A new method for the screening of solid-phase combinatorial libraries for affinity chromatography. J. Mol. Recognit. 17(3), 262–267.
Teng, S. F., Sproule, K., Hussain, A., and Lowe, C. R. (1999) A strategy for the generation of biomimetic ligands for affinity chromatography. Combinatorial synthesis and biological evaluation of an IgG binding ligand. J. Mol. Recognit. 12, 67–75.
Teng, S. F., Sproule, K., Husain, A., and Lowe, C. R. (2000) Affinity chromatography on immobilized “biomimetic” ligands synthesis, immobilization and chromatographic assessment of an immunoglobulin G-binding ligand. J. Chromatogr. B 740, 1–15.
Morrill, P. R., Millington, R. B., and Lowe, C. R. (2003) Imaging surface plasmon resonance system for screening affinity ligands. J. Chromatogr. B 793, 229–251.
Palanisamy, U. D., Winzor, D. J., and Lowe, C. R. (2000) Synthesis and evaluation of affinity adsorbents for glycoproteins: an artificial lectin. J. Chromatogr. B 746, 265–281.
Gupta, G. and Lowe, C. R. (2004) An artificial receptor for glycoproteins. J. Mol. Recognit. 17(3), 218–235.
Disley, D. M., Morrill, P. R., Sproule, K., and Lowe, C. R. (1999) An optical biosensor for monitoring recombinant proteins in process media. Biosens. Bioelectron. 14, 481–493.
Sproule, K., Morrill, P., Pearson, J. C., et al. (2000) New strategy for the design of ligands for the purification of pharmaceutical proteins by affinity chromatography. J. Chromatogr. B 740, 17–33.
Soto Renou, E. N., Gupta, G., Young, D. S., Dear, D. V., and Lowe, C. R. (2004) The design, synthesis and evaluation of affinity ligands for prion proteins. J. Mol. Recognit. 17(3), 248–261.
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Roque, A.C.A., Gupta, G., Lowe, C.R. (2005). Design, Synthesis, and Screening of Biomimetic Ligands for Affinity Chromatography. In: Zanders, E.D. (eds) Chemical Genomics. Methods in Molecular Biology™, vol 310. Humana Press. https://doi.org/10.1007/978-1-59259-948-6_4
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DOI: https://doi.org/10.1007/978-1-59259-948-6_4
Publisher Name: Humana Press
Print ISBN: 978-1-58829-399-2
Online ISBN: 978-1-59259-948-6
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