Abstract
Bacteriophage-based vectors, such as λ have been useful in creating gene llbrarles and in the expression of proteins However, a number of techniques used in molecular biology require DNA to be ina single-stranded form, such as chain termmatlon sequencing, ollgonucleotlde-directed mutagenesis, and certain probe synthesis strategies A small number of naturally occurring viruses produce single-stranded DNA as part of their life cycle, these include M13, fl , and fd, and a number of them have been mampulated and adapted as cloning vectors for the purpose of single-strand DNA production
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© 1998 Humana Press Inc , Totowa, NJ
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Rapley, R. (1998). M13 and Phagemid-Based Cloning Vectors. In: Rapley, R., Walker, J.M. (eds) Molecular Biomethods Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-59259-642-3_17
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DOI: https://doi.org/10.1007/978-1-59259-642-3_17
Publisher Name: Humana Press
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