Abstract
Nucleobases with attached side chains have been incorporated into oligodeoxynucleotides (ODNs) to achieve a number of goals. The functionalized side chains have been used to attach reporter groups (protein ligands as well as fluorescent moieties) and metal-chelating groups. Electrophilic and photochemical alkylating functions have been attached to react with the other strand(s) in a duplex (or triplex).
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© 1994 Humana Press Inc., Totowa, NJ
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Meyer, R.B. (1994). Incorporation of Modified Bases into Oligonucleotides. In: Protocols for Oligonucleotide Conjugates. Methods in Molecular Biology, vol 26. Humana Press. https://doi.org/10.1007/978-1-59259-513-6_2
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DOI: https://doi.org/10.1007/978-1-59259-513-6_2
Publisher Name: Humana Press
Print ISBN: 978-0-89603-252-1
Online ISBN: 978-1-59259-513-6
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