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Protecting Groups in Oligonucleotide Synthesis

  • Protocol
Protocols for Oligonucleotide Conjugates

Part of the book series: Methods in Molecular Biology ((MIMB,volume 26))

Abstract

A biopolymer is synthesized by assembling monomeric or oligomeric blocks. Each block features at least a nucleophilic and an electrophilic function, i.e., the α-amino and the carboxylic functions for peptides, the 5′-OH and the 3′-function (phosphate, phosphoramidite, or phosphonate), for nucleotides. The nucleophilic and electrophilic sites are linked together at the coupling step. Protection is a necessity. It guarantees the chemoselectivity of coupling and the solubility of synthons in organic solvents.

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  1. Laboratoire de Biochimie Physique et des Biopolymères, Louvain-La-Neuve, Belgium

    Etienne Sonveaux

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© 1994 Humana Press Inc., Totowa, NJ

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Sonveaux, E. (1994). Protecting Groups in Oligonucleotide Synthesis. In: Protocols for Oligonucleotide Conjugates. Methods in Molecular Biology, vol 26. Humana Press. https://doi.org/10.1007/978-1-59259-513-6_1

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  • DOI: https://doi.org/10.1007/978-1-59259-513-6_1

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-252-1

  • Online ISBN: 978-1-59259-513-6

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