Abstract
Unlike laboratory processing, large-scale methods are primarily concerned with efficiency and economics of scale. In handling biological materials there is constant “negotiation” between the engineer and chemist. The former is primarily concerned with efficient heat and mass transfer—because of its efficient utilization of energy, whereas the latter is concerned with maintenance or selective modification of the protein conformational state. As we will see, both criteria are not always optimally achieved. Furthermore, the transient and nonuniform conditions in large-scale plants have not been mathematically modeled, so that most progress still occurs by a combination of past experience and empiricism. Nonetheless, these derived guidelines are of value and lead to avoidance of the most obvious mistakes.
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© 1988 Humana Press Inc.
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Lillford, P.J. (1988). Large-Scale Methods for Protein Separation and Isolation. In: Franks, F. (eds) Characterization of Proteins. Biological Methods. Humana Press. https://doi.org/10.1007/978-1-59259-437-5_16
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DOI: https://doi.org/10.1007/978-1-59259-437-5_16
Publisher Name: Humana Press
Print ISBN: 978-0-89603-109-8
Online ISBN: 978-1-59259-437-5
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