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Methods of Protein Microcharacterization pp 381–401Cite as

Polypeptide Sequence Analysis Using Gas Chromatography-Mass Spectrometry

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Part of the book series: Biological Methods ((BM))

Abstract

Polypeptide sequence analyses have been carried out using a variety of chemical and enzymatic technologies. Although the most common is the Edman procedure, in many cases methodologies involving gas chromatography-mass spectrometry (GC-MS) may be the preferred route for these structure determinations. For example, a blocked polypeptide N-terminus is not a barrier to structure determinations as it is in the Edman technique. Also, Ser and Thr residues are always identifiable by GC-MS technologies, and C-terminal sequence determinations are as readily accessible as N-terminal analyses when GC-MS technologies are employed. In addition, unusual amino acid residues and posttranslational modifications to the polypeptide can be determined by GC-MS methodologies for sequence analysis.

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References

  1. Krutzsch, H. C. (1983) Methods in Enzymology 91, 511–524.

    Article  PubMed  CAS  Google Scholar 

  2. Krutzsch, H. C. and Pisano, J. J. (1977) Methods Enzymol. 47, 391–404.

    Article  PubMed  CAS  Google Scholar 

  3. Krutzsch, H. C. and Pisano, J. J. (1978) Biochemistry 17, 2791–2797.

    Article  PubMed  CAS  Google Scholar 

  4. Tondeuer, Yves, personal communication.

    Google Scholar 

  5. Kira, J. I., Deibler, G. E., Krutzsch, H. C., and Martenson, R. E. (1985) J. Neurochem. 44, 134–142.

    Article  PubMed  CAS  Google Scholar 

  6. Fairwell, T., Krutzsch, H., Hempel, J., Jeffrey, J., and Jörnvall, H. (1984) FEBS Lett. 170, 281–289.

    Article  PubMed  CAS  Google Scholar 

  7. Deibler, G. E., Martenson, R. E., Krutzsch, H. C., and Kies, M W. (1984) J. Neurochem. 43, 100–105.

    Article  PubMed  CAS  Google Scholar 

  8. Rao, D. N., Rudikoff, S., Krutzsch, H., and Potter, M. (1979) Proc. Natl. Acad. Sci. USA 76, 2890–2894.

    Article  PubMed  CAS  Google Scholar 

  9. Pawlita, M., Potter, M. and Rudikoff, S. (1982) J. Immunol. 129, 615–618.

    PubMed  CAS  Google Scholar 

  10. Yarmush, M. L., Krutzsch, H. C., and Kindt, T. J. (1980) Mol. Immunol 17, 319–326.

    Article  PubMed  CAS  Google Scholar 

  11. Henderson, L. E., Krutzsch, H. C., and Oroszlan, S. (1983) Proc. Natl Acad. Sci. USA 80, 339–343.

    Article  PubMed  CAS  Google Scholar 

  12. Marasco, W. A., Phan, S. H., Krutzsch, H., Showell, H. J., Feltner, D. E., Nairn, R., Becker, E. L., and Ward, P. A. (1984) J. Biol. Chem. 259, 5430–5439.

    PubMed  CAS  Google Scholar 

  13. Biemann, K. (1980) Biochemical Applications of Mass Spectrometry, Supp. I, 469–525.

    Google Scholar 

  14. Carr, S. A., Herlihy, W. C., and Biemann, K. (1981) Biomed. Mass Spectrom. 8, 51–61.

    Article  CAS  Google Scholar 

  15. Herlihy, W. C., Anderegg, R. J., and Biemann, K. (1981) Biomed. Mass Spectrom. 8, 62–69.

    Article  PubMed  CAS  Google Scholar 

  16. Herlihy, W. C. and Biemann, K. (1981) Biomed. Mass Spectrom. 8, 70–77.

    Article  CAS  Google Scholar 

  17. Carr, S. A., Hauschka, P. V., and Biemann, K. (1981) J. Biol. Chem. 256, 9944–9950.

    PubMed  CAS  Google Scholar 

  18. Kelley, J. A., Nau, H., Forster, J.-J., and Biemann, K. (1975) Biomed. Mass Spectrom. 2, 313–325.

    Article  CAS  Google Scholar 

  19. Haas, G. M., Nau, H., Biemann, K., Grahn, D. T., Ericsson, L. H., and Neurath, H. (1975) Biochemistry 14, 1334–1342.

    Article  Google Scholar 

  20. Samy, T. S. A., Hahm, K.-S., Modest, E. J., Lampman, G. W., Kentmann, H. T., Umezawa, H., Herlihy, W. C., Gibson, B. W., Carr, S. A., and Biemann, K. (1983) J. Biol Chem. 258, 183–191.

    PubMed  CAS  Google Scholar 

  21. Nau, H. and Biemann, K. (1976) Anal. Biochem. 73, 154–174.

    Article  PubMed  CAS  Google Scholar 

  22. Carr, S. A. and Biemann, K. (1980) Biomed. Mass Spectrom. 7, 172–178.

    Article  PubMed  CAS  Google Scholar 

  23. Herlihy, W. C., Kidwell, D., Meeusen, B., and Biemann, K. (1981) Biochem. Biophys. Res. Comm. 102, 335–340.

    Article  PubMed  CAS  Google Scholar 

  24. Hudson, G. and Biemann, K. (1976) Biochem. Biophys. Res. Comm. 71, 212–220.

    Article  PubMed  CAS  Google Scholar 

  25. Khorana, H. G., Gerber, G. E., Herlihy, W. C., Gray, C. P., Anderegg, R. J., Nihei, K., and Biemann, K. (1979) Proc. Natl Acad. Sci. USA 76, 5046–5050.

    Article  PubMed  CAS  Google Scholar 

  26. Herlihy, W. C., Royal, N. J., Biemann, K., Putney, S. D., and Schimmel, P. R. (1980) Proc. Natl Acad. Sci. USA 77, 6531–6535.

    Article  PubMed  CAS  Google Scholar 

  27. Hoben, P., Royal, N., Cheung, A., Yamao, F., Biemann, K., and Soil, D. (1982) J. Biol Chem. 257, 11644–11650.

    PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. National Cancer Institute, Bethesda, Maryland

    Henry C. Krutzsch

Authors
  1. Henry C. Krutzsch
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Editor information

Editors and Affiliations

  1. Beckman Research Institute of the City of Hope, Duarte, California

    John E. Shively

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© 1986 The Humana Press Inc.

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Krutzsch, H.C. (1986). Polypeptide Sequence Analysis Using Gas Chromatography-Mass Spectrometry. In: Shively, J.E. (eds) Methods of Protein Microcharacterization. Biological Methods. Humana Press. https://doi.org/10.1007/978-1-59259-436-8_15

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  • DOI: https://doi.org/10.1007/978-1-59259-436-8_15

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-090-9

  • Online ISBN: 978-1-59259-436-8

  • eBook Packages: Springer Book Archive

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